Vertices v 1, v 2, …,v m in V are connected in sequence into a Hamiltonian cycle.
For effective performance, WSPs should be connected in a sequence of three or more with waste matter to be transferred from the anaerobic to the facultative pond and finally to the aerobic pond.
Given any two nodes i and j of a network, they are said to be connected in case there is a sequence of edges extending from one of those nodes to the other, possibly passing through several relay nodes.
For SP, the maximum connection limit was ignored, forcing all sequences to be connected in a single network.
Thus, for this analysis, we used only those sets of sequences that could be connected in a parsimony network with a connectivity limit of 90%.
If two assembled sequences had high similarity (e-value < 10−10), they were connected in the sequence similarity graph.
Cathode and anode were connected in alternate sequence.
Each loop node (HP, IL, ML, and EXT) is connected, in 5' to 3' sequence order, to all UPI nodes which correspond to intervals of unpaired bases associated to the loop, and to all BP nodes which correspond to stem-terminating base-pairs adjacent to the loop.
All pairs of proteins were aligned using the EMBOSS needle program [ 23] and those with more than 30% sequence identity were connected in the paralogy network (see Methods).
The second strategy uses fusion gene vectors, whereby the two genes are connected in such a way that their coding sequences are in the same reading frame.
