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The C-terminus of ubiquitin is able to form covalent bonds with other proteins and once a single ubiquitin moiety is bound, it can be conjugated with additional ubiquitin molecules in a process called poly-ubiquitination [ 12, 13].
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AXT was conjugated with Epoxy-activated Sepharose 6B (GE Healthcare Korea, Seoul, Korea).
At this stage, the NPs were conjugated with the antibody through electrostatic interactions.
These LCFAs were conjugated with non-esterified fatty acid (NEFA -free BSA (Sigma-Aldrich, a7030).
All of these oligosaccharides were conjugated with polyacrylamide (PAA) and were biotinylated.
Before secretion, primary bile acids are conjugated with either glycine or taurine within the liver to form the conjugated bile acids, commonly known as bile salts.
Secondary antibody was conjugated with Cy5.
Secondary antibodies were conjugated with Cy3 or Cy5.
All were conjugated with phycoerythrin fluorochrome (PE).
Peptide substrate was conjugated with AMC.
The serum is conjugated with FITC for use in direct IF.
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