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Human cell line contamination can be confirmed using validated STR markers [ 17].
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All measures are validated.>> Lifetime and current psychiatric diagnosis will be confirmed using the psychosis and mood components of the Structured Clinical Interview for DSM-5 [ 16, 17].
Quantitative improvement in IAH was confirmed using the validated Clarke questionnaire [ 42] in addition to restored symptomatic response to clamp-induced hypoglycaemia.
DOI: http://dx.doi.org/10.7554/eLife.08827.017 10.7554/eLiFigure27 figuregure 7—figure supplement 2. Morpholino effects seen in this paper that have not previously been validated were confirmed using a second morpholino designed in a location distinct to the site of the first morpholino.
Binding of NEMO to several interactors, including CALB1, CDK2, SAG, SENP2 and SYT1, was confirmed using GST pulldown assays and coimmunoprecipitation, validating the initial screening approach.
To visualize Oct-4 mRNA in live stem cells, molecular beacons were designed, synthesized and validated, and the detection specificity was confirmed using control studies.
The differentially expressed genes were validated using real-time RT PCR and western blotting; and the functional effect of one induced gene was confirmed using siRNA technology.
This was confirmed using TBR1 null mice.
Convergence was confirmed using AWTY [67].
Protein purity was confirmed using SDS-PAGE.
Equal loading was confirmed using β-actin.
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