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Their physiological expression remains to be confirmed using specific antibodies.
The short fimbrial structures observed in the afimbriate strain overexpressed with ExPEC adhesin I will have to be confirmed using specific antibody and immunogold staining in the future; however, this study provides preliminary evidence for the fimbrial structures of this newly identified fimbrial adhesin.
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The extent of ALX expression in stably transfected HEK293 cells was confirmed using specific primer sequences designed against the protein coding region of the human ALX receptor (Figure 1B).
The defect in TLR2 and NOD2 in the respective KO mice used was confirmed using specific ligands, Pam3Cys-Ser- Lys)4 (Pam3Cys-Ser- Lysthylmuramyl-L-alanyl-D-iso-glutamine hydrate (MDPam3Cys-Ser- Lys using lipopolysaccharide (LPS) as positive control (data not shown).
The defect in TLR2 and NOD2 in the respective KO mice was confirmed using specific ligands, respectively Pam3Cys-Ser- Lys)4 (Pam3Cys-Ser- LysPam3Cys-Ser- Lysem, Germany) and N-Acethylmuramyl-L-alanyl-D-iso-glutamine hydrate (MDP, at 100 µg per ml, Sigma, ST-Louis, MI, USA) while LPS (at 100 ng per ml, Sigma) was used as positive control (data not shown).
Again binding was confirmed using specific and non-specific competitors.
Results were confirmed using specific anti-Myh6 and anti-MyBP-C3 antibodies (Fig 4D).
The presence of transgenes in the lines was confirmed using specific TaSK1 and TaSK2 primers (Additional file 7).
Isolate identification was confirmed using specific cards processed by a VITEK 2 instrument (bioMerieux, Marcy l'Étoile }France).
Presence for the transgenes in the severe dwarf lines was confirmed using specific primers for either TaSK1 or TaSK2 (Additional file 7).
This would be confirmed using more specific MAP diagnostic methods such as faecal culture.
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