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This could also be confirmed using quantitative real time PCR.
The induction of IFN-γ gene expression could be confirmed using quantitative RT-PCR (data not shown).
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All endotracheal tube positioning was confirmed using quantitative waveform capnography.
This decrease in PKG expression was confirmed using quantitative RT-PCR and western blotting of matched colon specimens from normal epithelium and tumor tissue, and also in colon derived cell lines where luciferase reporter analysis revealed that the decreased expression occurred at the transcriptional level.
This was confirmed using quantitative RT-PCR (qRT-PCR).
These results were confirmed using quantitative RT-PCR (Figure 3B).
These results were confirmed using quantitative real-time PCR analysis (Fig. S4).
Knockdown was confirmed using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) methods described previously [29].
This knockdown was confirmed using quantitative real-time PCR (Fig. S1).
Knockdown of the PRKCI mRNA was confirmed using quantitative RT-PCR.
This was shown in proliferative assays using various ratios of splenocytes versus MSCs and was confirmed using quantitative assays for specific immunosuppressive mediators.
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