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While the Norwegian cohort is entirely referral-based, diagnoses could be confirmed using either pathology records or records from the Norwegian Cancer Registry.
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Microarray data was confirmed using either total or amplified RNAs from original samples.
Post-pyloric placement was confirmed using either abdominal radiograph, scintigraphy and/or transmucosal potential difference [ 22].
All the LGV cases were confirmed using either real-time PCR of RFLP-PCR as were non-LGV CT controls.
Novel sequence variants identified in regions previously investigated were confirmed using either bidirectional Sanger sequencing or pyrosequencing.
Specific interactions between primers and target genes were confirmed using either NCBI BLAST http://blast.ncbi.nlm.nih.gov/Blast.cgi) or BlastView (http://uswest.ensembl.org/index.html.html
Zygosity of twins was confirmed using either 16 short tandem repeat (STR) markers (15 autosomal markers and 1 sex-determining marker) (67 %) or a self-administered zygosity questionnaire (33%%), which showed >90%% accuracy [ 44].
This needs to be confirmed using rice lines that are either mutated or knocked down for the JA pathway.
Their physiological expression remains to be confirmed using specific antibodies.
Similarly, deaths could be confirmed using ONS.
Presence for the transgenes in the severe dwarf lines was confirmed using specific primers for either TaSK1 or TaSK2 (Additional file 7).
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