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All anti-Ro and anti-La activities in our sera detected by ELISA could be confirmed using alternative biochemical techniques.
Thus, genes identified as differentially expressed in such initial discovery efforts need to be confirmed using alternative methods and larger sample sizes before they can be considered validated and advanced for testing as early detection markers.
We cannot explain this discrepancy, but it may be related to the use of different experimental designs; also, the proposed binding of EDCs to the COX enzymes should be confirmed using alternative experimental methods.
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Overproliferation phenotypes were confirmed using alternative secondary/tertiary RNAi lines.
This molecular heterogeneity has recently been confirmed using alternative gene expression analysis methods [ 34].
Therefore, unexpected positive results, such as in this case, should always be confirmed using an alternative test before conclusions can be drawn from the results [ 23, 24].
These results were confirmed using an alternative cell viability assay (MTT; data not shown).
The correlations of N-linked glycosylation with protein disorder have also been confirmed using the alternative tool POODLE for predicting protein disorder (Supplementary Fig. S5B).
The ancestral nature of haplotype BR25 was confirmed using an alternative method of haplotype network construction [ 34], which pinpoints the haplotype that is most probably the ancestral within a network.
I would feel much more comfortable if the data was confirmed using an alternative mode of binding these proteins (or at least Cdc24) peripherally to the plasma membrane, for instance through an amphipathic helix, a C2 or a PH domain.
This is confirmed using WGCNA as an alternative analysis method to identify genes associated with line differences.
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CEO of Professional Science Editing for Scientists @ prosciediting.com