This hypothesis was confirmed through direct visualization using AFM.
Germline transmission was confirmed through direct sequencing of Smn exon 7 PCR products (Figure 1C).
Gene orientation and homology of reference, rs11045819 (T155P), rs2306283 (D130N), and rs4149056 (V174A) SNPs1B1 SNPs were confirmed through direct full length sequencing of clones prior to experimentation.
A similar distribution of ω is observed for the subset of new microRNA sequences that were confirmed through direct association with Ago2 (Figure 3D, blue).
Copy numbers at each VNTR locus was confirmed through direct sequencing using DNA extracted from kidney tissues of challenged fish as templates (Table 4).
The presence of cattle, goats and rabbits was confirmed through direct visual observation of the animals or by the presence of characteristic faeces.
In terms of female-biased variability however, it is important to note that these relationships have not been confirmed through direct investigation of variation in female transcription between genotypes, something we aim to address in the current study.
The correct localization of the CpG island and the co-linearity of the UTR sequence with the FHIT coding sequence were confirmed through direct sequencing of PCR fragments obtained using respectively genomic DNA and cDNA as templates.
This will be confirmed through the biopsy.
