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By measuring OD it could be confirmed that cell growth was indeed promoted together with enhanced glucose consumption when increasing pH at high acetic acid concentrations (Figure 3).

It has also been confirmed that cells with regulatory function constitutively express high levels of the FoxP3, but this is an intracellular transcription factor, so isolation of live human cells based on FoxP3 staining is not possible.

Additionally, it was confirmed that cells can be cryopreserved at -20°C or with liquid nitrogen for several weeks before staining.

It was confirmed that cells could survive for 9 days on lysogeny broth (LB) agar plates containing 10 12 g/L n-butanol (Additional file 1: Figure S4A).

Furthermore, it must also be confirmed that the cell death observed after RF treatment is due only to drug release and not to magnetic hyperthermia produced by the magnetic nanoparticles.

It was confirmed that all cell lines were the same as the cells registered in Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan), by the comparison with the database of JCRB cell bank.

It has also been confirmed that stem cell differentiation can be enhanced by both native and chemically modified TMV through regulating the gene's expression [9 11].

Biofilm formation may be a survival strategy for starved bacteria, and it has been confirmed that increased cell adhesion is often correlated with nutrient limitation [ 23].

It was confirmed that the cells maintained their viability well after the manipulation process including photo-induced cell capturing.

First, it was confirmed that effector cells from control and HIV+ subjects displayed comparable levels of proliferation, both at the high and low CD3/28 mitogenic bead∶cell stimulation ratios of 2∶1 and 0.2∶1 (Fig. 2A and 2B respectively).

Thus, it was confirmed that these cancer cells express more TfR than normal cell lines, as reported previously (4-12).