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Still, in silico high scoring epitope candidates have to be confirmed for binding to HLA-A*0201 HLA-A*0201
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Once the samples were confirmed for binding antibodies, their neutralizing capability was assessed using a biological assay.
Specific binding could be confirmed for nearly all selected antibody fragments except for the clones CHN24-2-A1 CHN24-2-A1 CHN24-2-A1
In a common tier based approach, samples identified as ADA screen positive are confirmed for the binding specificity of the antibodies to the drug molecule via a confirmatory assay.
The specificity of Twist1 binding was confirmed for tissue-specific targets, while Twist1 binding in multiple tissues was detected for shared target genes, including four genes that represent core targets of Twist1 in multiple cell types.
The generated antibodies were confirmed for their specificity by binding to type-specific HDAgs expressed in DNA-transfected Huh-7 hepatoma cells.
ChIP-on-chip results were confirmed for 10 of the 13 binding fragments using ChIP-PCR.
The antigen binding was confirmed for all Yumabs.
CREB binding was confirmed for all loci supported by multiple GSTs.
Subsequently the function of CM1 in γ-TuSC binding was confirmed for Mto1, centrosomin (Drosophila), and CDK5RAP2 (human) (Zhang and Megraw, 2007; Fong et al., 2008; Samejima et al., 2008; Choi et al., 2010).
High binding affinities were confirmed for all 30 mutants containing loop sequences that differed from those of wild-type.
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