To ensure that the base substitutions introduced to create the [GT/CA] series did not disrupt HSV-tk protein function and subsequent selection, the HSV-tk phenotype was confirmed by selective plating in the presence 2 μg/ml trimethoprim, an antibiotic that selects for bacteria carrying a wild-type, plasmid-encoded HSV-tk gene.
HSV-tk function of all constructs was confirmed by selective plating in the presence of trimethoprim, which selects for plasmids bearing wildtype plasmid HSV-tk, as described (Eckert et al. 1997).
The significant role of specific negative regulators in reducing the IL-8 inflammatory response was confirmed by selective knockdown experiments using primary enterocytes isolated from fetal intestine (Figure 5A).
EDH dependence on SKCa and IKCa in the mesenteric artery of normal adult rats was confirmed by selective SKCa and IKCa block [3], [7], [40], and supported via direct endothelial SKCa and IKCa activation with CyPPA and 1-EBIO, and subsequent apamin/TRAM-34 block [16], [28], [29], [30], [41].
This was attributed to spectral overlap of the etheno H6 and H7 protons, which was confirmed by selective deuteration of the H7 proton (18).
The described assay is reproducible, sensitive, can be easily performed and is able to give a presumptive identification of the causal pathogen, which could be confirmed by culture techniques using selective media.
This selective pressure may be confirmed by future studies, but the observation that essential genes (whose numbers of the proteins in monomeric form alone lie on the noise floor) are more likely to multimerize than non-essential ones, is already tentative evidence for the existence of this pressure.
Satisfactory separation performance of PPG is confirmed by the selective discrimination and isolation of β-lactoglobulin from complex biological sample matrixes, e.g., milk whey.
The correspondence between these mounds and the buried nanostructures is confirmed by posterior selective strain-driven formation of new nanostructures on top of them, when the distance between the buried and the superficial nanostructures is short enough (d = 25 nm).
The fact that polarized neuronal cells and multipolar cells actually belong to two distinct populations of newlyborn cerebellar cortical cells was confirmed by their selective expression of distinct nuclear transcription factors: the PSA-NCAM+/DCX+ cells (Figure 2G,H,K) were immunoreactive for Pax2 [17] [17], and were consistently negative for both Sox2 and Olig2 (Figures 2 and 7A).
