To date, data linkage has been conducted with specific health data (such as discharge coding from hospitalisations, immunisation records and pharmacy data) and there is also scope to link with education data.
As individual studies are conducted with specific aims in mind, the types of data reported in each study may vary and some may contain a measurement type or variable determination unique to that study.
To address the question of the concept's application in the political practice of the European Union, an analysis of currently existing EU policies has been conducted with specific focus on implemented nexus thinking.
A comparative study of the ability of microparticulate zerovalent iron (mZVI) and nanoparticulate zerovalent iron (nZVI) to oxidize a target compound (in this study, 14C-labelled formate) under aerobic conditions has been conducted with specific consideration given to differences in reaction mechanisms.
Nested polymerase chain-reactions (PCR) were conducted with specific primers.
Immunohistochemical examinations were conducted with specific antibodies.
To verify the insertion of the designed DNA fragment, PCR was conducted with specific primers using the plasmid DNA as template.
Immunoblotting was conducted with specific antibodies against phospho-AktSer473, total Akt, phospho-GSK3βSer9, total GSK3β, phospho-Erk1/2Thr202/Tyr204 and total Erk1/2 (Cell Signaling, Boston, MA, USA).
RT and Q-PCR analysis for liver UGT1A gene products were conducted with specific oligonucleotide primers for each gene (Fig. 4A).
However, four of these studies were conducted with specific populations, including adolescents with cerebral palsy [ 30], obesity [ 31], disadvantaged backgrounds [ 33], or within a context of a parent-child agreement [ 32] and therefore provide limited generalizability to broader adolescent populations.
