Antisense ODNs were designed to be complementary to sequences in the 5'- and 3'-untranslated regions of the human OP-1 messenger RNA (mRNA) sequence (XM_030621, National Center for Biotechnology Information (NCBI)) as described [ 3].
PPMO 5UP1 that is complementary to 5′ terminus of PRRSV genome was paired with 4P1 or 7P1 that are complementary to sequence in the translation initiation regions of ORFs 4 and 7, respectively.
The core region is complementary to sequence flanking the SNP and has a low melting temperature.
The antisense oligonucleotide is complementary to sequence in the rat preproghrelin 3' UTR in exon 4 (exon 4* of putative rat ghrelinOS transcripts).
This trend is complementary to sequence-conserved residues as we noted previously for another centrality measurements [11].
The primers were complementary to sequences which flank the inserts and do not include the regions which encode GST or the GAL promoter.
The primers used for the RT-PCR reaction shown in Figure 1D were complementary to sequences flanking intron 2. The same primers were used on genomic DNA (gDNA) to assess intron removal.
cNot applicable; these primers are complementary to sequences inside the IS 3 elements.
These primers carry extensions, which are complementary to sequences 30 bp upstream (oAfhapX-S1) and downstream (oAfhapX-S2) of the hapX stop codon (TGA), thereby generating a 132-bp-long PCR product.
However, highly conserved regions identified in 13 yeast snoRNAs (snR13, snR39, snR47, snR48, snR61, snR64, snR70, snR73, snR75, snR76, snR87, snR190 and U18) are complementary to sequences immediately upstream or downstream of the rRNA methylation site.
