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When the target miRNAs are added simultaneously, these cDNA (cDNA-1 and cDNA-2) can be competed off from the cDNA∖probe duplex to form a cDNA∖RNA heteroduplex.
SAM binding by Ash2L was UV-dependent and could be competed off by excess amount of unlabeled SAM (1000 fold, ∼0.35 mM) (Fig. 3A).
Binding to the wildtype probe could be competed off by the addition of a 100-fold molar excess of a non-radiolabelled wildtype probe to the nuclear extract (lane 3) while the addition of an excess of mutated non-radiolabelled probe had no effect on binding activity (lane 4) confirming that binding to the −216 Ets site was specific.
The nuclear extracts from either ARPE-19 cells or from FHL124 cells showed a strong binding to CREB site (lane 1, 6 of Fig. 4B), this binding can be competed off by the cold CREB oligo (lane 2, 7 of Fig. 4B) but not by the mutant oligo (lane 3, 8 of Fig. 4B).
This interaction could be competed off with unlabelled oligo and supershifted using the YB-1 antibody.
We assessed whether other polysome-associated proteins, many of which may have a role in translational regulation, can also be competed off polysomes by kcRNA.
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The binding of iRGD-MBs on the endothelial cells was competed off by pre-incubation with the anti-αv or anti-β3 antibody (p < 0.01).
The binding assay showed that the binding of FITC-SERPINA3K to rMC-1 cells was competed off by excess concentrations of unlabeled SERPINA3K, but not by BSA (Fig. 7B), suggesting that the binding of SERPINA3K to Müller-derived rMC-1 cells is reversible and specific.
The Sryα binding is competed off with unlabeled Sry DNA (lane 7).
While the increased prostasin can activate matriptase, it is also possible that the cognate serine protease inhibitor(s) for both proteases, e.g., HAI-1, is competed off matriptase by the increased prostasin.
The record was even more remarkable, given the fact she was competing off a 12-stride approach as opposed to her usual 16 strides.
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