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To clarify to what extent the neurite terminals observed in PC12 cells can be compared to neuronal growth cones, we have analyzed the different morphologies of these terminals and the distribution of growth cone markers.
To clarify to what extent PC12 neurite terminals can be compared to neuronal growth cones, we have analyzed their morphology and protein distribution in fixed PC12 cells by immunocytochemistry.
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Results were compared to fresh cultures with no differences in cell viability, percentages of neuronal type or morphology observed.
In order to examine the neuronal processes that underlie conscious states, the neuronal activity associated with one state of consciousness (e.g., sleep) is compared to another (e.g., awake).
Though glial Fos expression was also observed in our experiment (Fig. 3B') and that of other investigators [36], the quantity of glial Fos expression was extremely small compared to neuronal population.
Glial nuclei were identified by their small size compared to neuronal nuclei and by their location either in the axonal sorting zone region of the antennal nerve where they are the only cell type present [9] or in the envelope surrounding each glomerulus [44], [60].
Intriguingly, also neuronal cells co-cultured with PGC-1α-overexpressing astrocytes were protected against an exogenous oxidative attack compared to neuronal cells co-cultured with control astrocytes.
As previously suggested, baseline rates and patterns of neuronal activity need to be compared across fairly large numbers of dystonia cases to draw conclusions concerning the relationship between mean firing rates, response to somatosensory input, or patterns of neural activity in GPi, and the phenotype, or etiology of dystonia.
In addition, the length of neuronal cilia was reduced in SBH compared to neurons in the overlying and adjacent normotopic cortex.
This effect could then be compared with the effect of attention on neuronal firing, and thereby assess whether attention employs normalization mechanisms (see Data analysis below).
Of note is that three internal controls were compared: the neuronal specific genes Enolase 2 (ENO2 Hs00157360_m1) and synaptophysin (SYP Hs00300531_m1) and the house keeping gene glyceraldehydes-3-phosphate dehydrogenase (GAPDH Hs00999905_m1).
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