Using immunohistochemistry the groups were compared for expression of apoptotic proteins: bcl-2, bcl-XL, bax, bak and survivin.
Urothelial tissues and cells from affected clinical subjects and asymptomatic controls have been compared for expression of proteins and mRNA.
mRNA sequence data obtained from Zfp42-GFPd2-high and Zfp42-GFPd2-low populations (Marks et al., 2012) were compared for expression level changes.
CPSF6, HNRPM, PFKP and TAF1C, together with HPRT, GAPDH, TBP, PBGD and CYPA, were compared for expression stability in the panel of breast cancer cell lines.
Expression information was available for 21,733 genes, 14,809 of which were associated with one of 22,290 450 K CGI (only those CGI in resorts previously classed by steepness were compared for expression).
Additionally, NAF and CAF were compared for expression of insulin-like growth factor II as a potential mediator of their effects on epithelial cell growth, by ELISA and by quantitative, real-time PCR.
When the different sample groups were compared for expression of these four genes, their pattern of expression accompanied the genomic alterations: SMAD2 was less expressed in the carcinomas, while EFNA1, BOP1 and STMN3 all showed an increased expression in the malignant tumor fractions; EFNA1 was also notably expressed in the A/C fractions.
Other samples may then be compared for the expression of this same gene.
Immunoprecipitates were probed with anti-HA (top blots) and total lysates were compared for HA expression and Myc expression to ensure that equal inputs were analyzed.
