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As an alternative to deorbit, some of them can be collected for storage and possible in-orbit recycling.
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Moreover, four blood samples were collected for storage (Table 1).
The interval length between visits ranged 4 8 months; serum samples were collected at all visits, however, cells were collected for storage at enrolment and 3 years after enrolment.
With these findings, we recommend that seeds be collected for germplasm storage and ex situ conservation of both the CZH and DHT populations.
One frozen blood sample will be collected for long-term storage for potential future analysis.
Blood was collected for parasite microscopy, storage on Whatman 3M filter paper and, at specified visits, for determination of the full blood count and biochemical parameters.
M3 seeds were collected for long-term storage and production of further material.
In addition, seeds were collected for long-term storage from some of the populations to which the individuals belonged.
As soon as a cytopathic effect became visible or after a maximum of seven days, the supernatants were collected for TNA extraction and for storage at −80 °C until further use.
Blood samples are collected for immunologic and virologic testing and for storage.
Two additional 10 ml Vacutainer lavender tubes are collected for extracting plasma for long-term storage in the biorepository; these tubes are also processed so that the remaining buffy coat and red cells can be stored and batch shipped to Prevention Genetics (Marshfield, WI) for DNA extraction and storage.
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