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Obvious cases can also be clustered manually.
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Clones were then clustered manually based on shared insertion sites, and any samples clearly derived from more than one clone excluded.
For visualization, columns (features) were clustered using hierarchical Ward clustering and manually rearranged if necessary.
The results were clustered by BLASTCLUST and each cluster was manually checked.
The base content of the array was clustered according to the published cluster file from the CHARGE consortium (30), while the custom content, comprising ∼25 000 variants, was manually assessed/clustered whenever GenTrain scores were <0.9.
a Unigenes were obtained using Phrap software and clustered manually.
SNP markers that were polymorphic between the B. napus and B. carinata parents (C genome) and between the B. napus and B. juncea parents (A genome) were retained, and SNP marker clustering manually checked.
Model species of interest could be manually clustered or automatically categorized into two groups of mammal and marine species clusters.
To do this, principal component (PC) analysis was used to determine the diversity of the image cube, convex geometry was used to find the spectrally pure pixels, and then these pixels were manually clustered in PC space.
The set of SNP markers used in this study was manually clustered in Genome Studio for these genotypes previously (see [ 25]) and markers that showed more than three genotype clusters or were heterozygous in the control genotypes (indicative of cross-amplification between the A and C genomes) were removed.
Compounds retrieved from the screening collections were manually clustered.
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