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Therefore they can generally be closed by methods such as primer walking.
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It is clear that this is a gap in research that needs to be closed, by devising mechanisms to generalize navigation methods to be applied over a wide range of mobile platforms.
These gaps were closed by direct PCR methods, and the scaffolds were joined together into the first assembly sequences over 3 947 535 bp in length, covering 99.09% of the genome.
The low quality regions and gaps were closed by a variety of finishing methods [1], and the final genome was a single circular chromosome containing 1,895,727 base pairs (bp) (GenBank accession CP000437).
Physical gaps were closed by primer walking, PCR, and multiplex PCR methods.
All were closed by 1836.
Yet 20 years later, Philips still hasn't learned that lesson: in the digital age, you can flourish only by using openness and pairing it with a closed method, not by being closed all the time – and especially not by moving the digital goalposts to close formerly open areas.
The answer will vary depending on the type of policies issued by the fund that is closing, the method of closure and the way it will be run in future.
It's close by.
"I used to call for a transitional period by peaceful and democratic methods, but the regime is closing that door.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com