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In 1996, Dolly the sheep became the first animal to successfully be cloned using this method.
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While the region encoding PAP85 described here was 5.5 kb, sequences of up to 45 kb have also been cloned using this system (data not shown).
Dolly was the first mammal to be cloned using DNA taken from an adult cell.
The insert was cloned using the NheI and XbaI sites.
Dolly the sheep was cloned using the process of somatic cell nuclear transfer (SCNT).
Two monkeys have been cloned using the technique that produced Dolly the sheep.
Purified PCR amplicons were cloned using the pGEM-T Easy vector system (Promega, Fitchburg, WI, USA).
Tmub1/HOPS was cloned using the Gateway™ system.
The spy0129 gene was cloned using the conventional ligation method.
PCR products were cloned, using the TOPO TA cloning kit for sequencing (Invitrogen).
Products were cloned using the TOPO TA cloning system following instructions of the manufacturer (Invitrogen).
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