Complementary DNA (cDNA) libraries can be cloned into various vectors, including cosmids or phagemids.
Then he inserts a single cell drawn from the tissue sample of the animal to be cloned into the genetically void egg and sets it down between the egg's outer wall and inner cytoplasm.
SCNT entails the transfer of the nucleus from a somatic (body) cell of the animal to be cloned into the cytoplasm of an enucleated donor egg (an egg cell that came from another animal and that has had its own nucleus removed).
More important, all genes in the obtained genomes can be cloned into expression vectors to produce proteins that can be used as antigens for diagnostic reagents or as immunogens for vaccine development.
Alternatively, they could be cloned into vectors suitable for direct vaccination of birds as nucleic acid vaccines.
Genes identified as potential virulence factors can be cloned into suitable vectors for expression of recombinant proteins.
Finally, they could be cloned into bacterial expression systems, which could then be isolated as recombinant bacteria, which could also be used to immunize birds.
These results indicated that a multi-integrase platform can be cloned into the defined locus on the HAC vector by the Cre/loxP system.
PCR products were double-digested with BglII and NotI prior to be cloned into the similarly double-digested pET-32a vectors.
The mutant cDNA sequences have been digested with EcoRI and NotI restriction enzymes, to be cloned into a previously digested pCDNA5/FRT.
In this way, different genes of interest can now easily be cloned into a universal shuttle vector and then into a BAC.
