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The data store can be cloned from: https://github.com/OpenTreeOfLife/phylesystem.
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FL clones were retrieved from the cDNA library used for Sanger sequencing and were cloned from pDNR-LIB into the pET28b vector (EMD Chemicals, http://www.emd-chemicals.ca) for expression.
Bph26 was cloned from indica variety, ADR52.
Rab3a was cloned from mouse brain cDNA.
Ferret NKCC1 was cloned from adult kidney.
All the MEXPRESS code (back-end, front-end and data processing) can be cloned or downloaded from this GitHub repository: https://github.com/akoch8/mexpress.
The verified clone was digested with Hind III and Eco RI and the DNA fragment was cloned into pCAMBIA1301 (http://www.cambia.org).org
NOD/ShiLtJ male kidney genomic DNA, obtained from the Jackson Laboratory, was cloned in pTARBAC2.1 and these clones are available from http://bacpac.chori.org/.org/
hkb (KM489061) was cloned using sequence data from our published early embryonic transcriptome (http://diptex.crg.es; gene ID: comp8024) (Jiménez-Guri et al., 2013).
More than 5000 different P450 genes have been cloned up to date (for details see: http://drnelson.utmem.edu/CytochromeP450.html).edu/CytochromeP450.html
A Bam file containing the hg19 mapping of the whole-genome sequencing of clone 6 can be downloaded from http://biorepo.epfl.ch/biorepo/public/public_link?sha1=3da4be0675e2a56b6d794b51e82ecad821891b6a.
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