First, they perform transcription by the "cap-snatching" mechanism, whereby short 5′ capped RNA fragments are cleaved from host cell mRNA by an endonuclease intrinsic to the RdRp and then used to prime synthesis of viral mRNAs (Morin et al., 2010; Plotch et al., 1981; Reguera et al., 2010; Reich et al., 2014).
We next determined the expression of FasL, a molecule known to be cleaved and released from the cell surface by MMP7 [4].
He can't be cleaved apart like that, not anymore.
Some of the Cfl1 proteins are cleaved and released from the cell wall to the ECM.
Pro-NTs may be secreted from cells (7) or cleaved intracellularly by furin or proconvertases to yield the mature C-terminal NT.
Chromatin was extracted from cells and cleaved by sonication or enzymatic digestion to obtain fragments of approximately 200 to 600 base-pairs, as confirmed by polymerase chain reaction using primers designed to amplify the IAP segments of interest.
Antibodies against cleaved Notch1 (N1ICD) were purchased from Cell Signaling (Saint Quentin en Yvelines, France); Puma, from Epitomics (Nanterre, France); and Bim and Noxa, from AbCam (Cambridge, UK).
Anti-Drp1 antibody was from BD (San Jose, CA); anti-hnRNP A1 was from Santa Cruz Biotechnologies Santaa Cruz, CA); anti-cleaved caspase-3 was from Cell Signaling Technology (Danvers, MA); anti-Actin antibody was from Millipore (Temecula, CA).
Anti-active caspase-3, anti-active caspase-7, anti-caspase-9 and anti-cleaved PARP were obtained from Cell Signaling Technology (Beverly, MA, USA).
Antibodies specific for Bax, Bak, Mcl-1, Bim, phospho-Bad, total Bad, Puma, Bcl-2, Bcl-XL, cleaved poly-ADP ribose polymerase (PARP), cleaved caspase-3, cleaved caspase-9, cleaved caspase-8, and cleaved caspase-7 were from (Cell Signaling, Danvers, MA, USA).
