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Undegraded proteasome substrates, including PMP22, can be cleared from cells through autophagy (Fortun et al., 2007; Lamark and Johansen, 2010).
Hirano bodies have also been reported to be extruded from neurons and model Hirano bodies can be cleared from cells through exocytosis [ 15, 25].
In addition to the detailed findings, the results overall support the conclusion that intracellular r-GV are susceptible to degradation and therefore can be cleared from cells that may phagocytose them.
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The interaction between the S. pombe cap methyltransferase Pcm1 and CDK9 is such that all detectable Pcm1 can be cleared from cell extracts by an immunoprecipitation with CDK9 [ 69].
Fluid was cleared from cells and fractionated by size exclusion chromatography and polyacrylamide gel electrophoresis.
Undegraded proteasome substrates are cleared from cells by the autophagy lysosomal pathway (Nijholt et al., 2011).
These observations are consistent with the proposal that expression in the periproctodeal region of the Strigamia embryo is highly dynamic, with transcripts being cleared from cells once every cycle of the pattern.
Reactive oxygen species are free radicals or reactive molecules containing oxygen atoms, such as superoxide or hydrogen peroxide, and are cleared from cells by the action of superoxide dismutase or glutathione peroxidase.
The suspension was cleared from cell debris by centrifugation at 8000 g for 20 min.
Pure cytosolic fractions were cleared from cell lysate by ultracentrifugation at 100 000 × g for 1 h at 4°C.
The supernatants were cleared from cell debris by centrifugation at 4500 x g for 1 hour, and subsequently centrifuged at 100,000 x g for 1 hour.
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