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What should be clarified of their start, however, is that the two games they have lost have been their only two against any of last season's top 7.
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Lysates were clarified of genomic DNA by passage through QiaShredder spin columns (Qiagen) and heated to 95°C for 5 min prior to separation on a 7.5% polyacrylamide gel.
The cell lysates were clarified of cellular debris by centrifugation (13 200 × g) for 10 min at 4 °C.
Cell lysates were clarified of cellular debris by centrifugation (10000xg) at 4*00BA*C for 10 min, and the supernatants were used in the biochemical assays.
Cell lysates were clarified of cell debris by centrifugation at 14,000x g for 5 min through a QIAshredder spin column assembly (Qiagen, Hamburg, Germany) at 4°C.
The mixture was incubated for 30 min on ice, combined with vortexing every 10 min. Cell lysates were clarified of cell debris by centrifugation at 14.000 × g for 5 min through a QIAshredder spin column assembly (Qiagen) at 4°C.
The mixture was incubated on ice for 30 min, combined with vortexing every 10 min. Cell lysates were clarified of cell debris by centrifugation at 14.000 g for 5 min through a QIAshredder spin column assembly (Qiagen, Hilden, Germany).
Supernatants were clarified of cell debris by centrifugation at 1,500× g for 10 min, ultracentrifuged at 100,000× g for 30 min at 4°C and stored at −80°C until use.
However, several issues need to be clarified for realization of the optimal use of TCZ.
The role of dogs in transmission in Poland cannot yet be clarified because of lack of data.
The causal effect could not be clarified because of the initial study design of the clinical trial for antihypertensive drugs.
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