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After design, the primer binding sites can easily be checked in Vector NTI to ensure the optimal position of each primer regarding to any regions of particular interest.
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As controls, inductions were checked in only E. coli BL21 (DE3) pLysS cells and the same cells containing only pET14b vector.
Details are checked in Sect.
To guarantee similarity between detected and referenced triangle, direction of vector product in relation to average direction of normal vectors is checked.
The transferred vector was checked by DNA sequencing.
The resulting vector was checked by sequence analysis.
The final and intermediate vectors were checked by restriction and sequencing.
All vector constructs were checked by DNA sequencing.
Neo-resistant clones were checked for correct integration of the targeting vector by PCR.
All vectors inserts were checked by sequencing by Genome express (France).
Sequences of unique clones were checked for the open reading frame (ORF) status in the T7 expression vector.
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