Collinearity among the variables in the multivariate model was checked and determined absent.
The cDNA library was diluted to 8 nM and this final concentration was checked and determined again by Quant-IT dsDNA HS Assay before Illumina sequencing.
The serum was stored in a 2 mL freezing tube from Greiner Bio-One GmbH Co., Germany, which was checked and determined to be free of any detectable PAH background.
Ethnicity was determined by checking patient information, and if it was not specified, first and last names were checked and ethnicity was determined by means of the combined name method [ 22].
Values more than 2SDs from each subject's own mean were checked and, when necessary, manually determined by visual and auditory inspection of the audio file.
The cooling performance of the acoustically determined enclosing structure is checked and confirmed through numerical heat flow analysis.
After equilibrium, the samples were visually checked and determined as being clear microemulsions, or emulsions, or gels.
The integrity and quality were checked by determining absorbance using the NanoDrop spectrophotometer ND-1000 (NanoDrop Technologies Inc., USA).
RNA purity was checked by determining the A260/A280 ratio, and RNA integrity was checked by agarose gel electrophoresis.
The data were checked for completeness and determined to be free of errors prior to statistical analysis.
