The roles of viral encoded proteins involved in the infection cycle of LCV have not yet been characterized due to the lack of reverse genetic tools.
Alternatively, some phenotypes will occur independently of the mutation being characterized due to background effects (e.g. the A/J strain has a high incidence of cleft lip/palate).
Indeed, knowledge about the perfect and most appropriate parameterization is often not available or easily obtainable when performing in-depth sequence analysis, and, sometimes the 'correct' parameters may never be precisely characterized due to the complex nature of the experiment.
*Thermodynamic parameters for some interactions could not be fully characterized due to relatively low affinity, limited solubility of protein and limited availability of ligand.
In general, the glucosamine was firstly eluted, and then, the dimer and trimer were sequentially characterized due to the adsorption strength difference to the stationary phase.
EWS-FLI1 is an intrinsically disordered protein and structural property of this protein is less characterized due to its insolubility.
These compounds were not isolated due to further degradation during chromatographic processing, and their structures were not characterized due to miss matched fragmentation pattern with the available structure according to the molecular formula in Dictionary of Natural Products.
Many plant miRNA gene families have been identified via sequencing and bioinformatic analysis, but, to date, only a small tranche of these have been functionally characterized due to a lack of effective forward or reverse genetic tools.
The HG2D and HG5 strains were not characterized due to reproductive problems.
