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However, these pads must be changed daily for ten days.
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Medium was changed daily for fresh mTeSR1.
ES media containing either dorsomorphin or DMSO was changed daily for three days.
For RA-D0 differentiation, cells were cultured in ES cell media with LIF instead of RA, and media was changed daily for 2 days.
Media were changed daily for ambroxol treated and control lines.
Culture medium was changed daily for an additional 4 days.
The medium was changed daily for three days.
The EDTA solution was changed daily for 3 days and maintained at 4 °C.
Selection media were changed daily for 4 days at which point only puromycin-resistant cells remained.
Differentiation medium was changed daily for the first 3 days, and every 12 h for the remaining 3 days.
Media and drug were changed daily for 5 days, then cells were counted and replated in six-well plates.
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