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It can be challenging to monitor changes in the concentration of specific molecules inside cells, but researchers recently developed a form of microscopy based on FRET (short for Forster resonance energy transfer) that can measure the levels of a second messenger molecule called cyclic di-guanylate (c-di-GMP) inside individual cells.
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However, owing to the current limitations in evaluating glaucomatous neurodegeneration, it is challenging to monitor the disease severity and progression objectively, and to design rational therapeutic strategies accordingly.
However, it is challenging to monitor the activity of the root-zone because roots are not visible from the soil surface, and root systems undergo spatial and temporal variations in response to internal and external conditions.
It is challenging to monitor and control a 14-year-old's behavior in every aspect of life, including TV watching.
It is challenging to monitor the growth rate of the above transformant cells in Avicel-mineral medium.
SPNs can be challenging to manage and costly to monitor with radiation exposure of repeat CT scans and potential morbidity of biopsy or surgical resection of benign nodules.
Thus, it is challenging to measure and monitor such processes under field conditions.
Hot spots are challenging to characterize, because regulatory monitors are designed and sited to monitor compliance with air-quality standards and are not ideally equipped to assess or capture local hot spot formation arising from mobile sources.
Due to the nonobvious symptoms contaminated by a large amount of background noise, it is challenging to beforehand detect and predictively monitor the weak faults for machinery security assurance.
It is challenging to devise generic indicators for monitoring and evaluating capacity building since each project is unique [ 28].
Safety was of cardinal concern, and three vaccinated volunteers were challenged first to monitor clinical symptoms and tissue response.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com