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Since disulfide bonds are often necessary for scFv folding, it can be challenging to express scFvs in the reducing environment of the cytosol.
Iron sulfur cluster containing proteins of bacterial origin are known to be challenging to express in eukaryotes due to differences in the complicated cell machinery responsible for the loading of the Fe S cluster onto the apoprotein (Benisch and Boles 2014).
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Due to their ease of propagation and growth in confined environments, HRCs have also been used in recent years in the synthesis of recombinant therapeutic proteins, especially those that have been challenging to express in bacteria, yeast and mammalian expression systems.
RBR domains are challenging to express in bacteria due to their multiple Zn-binding folds.
Every day, students were challenged to express their gained knowledge through a variety of media and to employ critical thinking skills while attempting to make connections across disciplines.
They were challenged to express Cre-dependant transgenes upon exposure to the same sets of CPP-Cre fusion proteins, in individual wells.
The research team did not want to oversimplify the many issues, varied experiences and potential contradictions likely to emerge from such a study and anticipated that the impact of drawing on student learning would be challenging to identify and express.
While it was challenging to encourage mothers to express themselves, adequate data was gathered for thematic analysis.
We were constantly challenged to express ourselves as a single taiko performer and a Kodo member, without the traditional happi coats and hachimaki headbands.
Other youngsters, accustomed to adults solving their every problem, may express resentment at being challenged to come up with solutions.
In creating soil layer models for our study site, we were challenged (i) to express vagueness of our soil data, while at the same time maintaining adherence to systematic classification principles, and (ii) to describe continuously the spatial distribution of soil materials and layers in three dimensions.
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