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To reduce false negatives that can be caused by sequence variability, a new universal primer pair was designed against a divergent sequence data set, targeting the open reading frame 4 (heat shock protein 70 homologue gene), and optimised for conventional one-step RT-PCR and one-step SYBR Green real-time RT-PCR assays.
The high expression levels of Gilz mRNA can also be caused by sequence variations in the 3'UTR, the promoter region, or any other region affecting transcription and translation efficacy, promoter activity or exon splicing.
The specific pathogenic features of L. ivanovii may be caused by sequence differences in virulence genes shared with L. monocytogenes or by differences in the gene content of these 2 species (1, 6 ).
Furthermore, the ability to assess dosage of amplicons allows users to catch some cases of allelic dropout that could be caused by sequence variants under terminal primers which would not be observable in overlapping amplicons.
Thus, artifactual "under-representation" of these regions would be caused by sequence divergence in the UTRs beyond the point of blast-detectable similarity and not by the actual bias against UTRs in our sequences.
Similar(55)
The high variability may be caused by sequencing (or alignment) artefacts (Ib) or truly reflect the species' genetic diversity (Ic).
A similar depletion of nucleosomes is detected for nontranscribed pseudogenes, which is likely to be caused by sequence-dependent nucleosome-inhibitory effect.
Random SNP marks are likely to be caused by sequencing errors.
The peak at 4.6 is likely to be caused by sequencing and statistical phasing errors.
This could be caused by sequencing error or automated annotation [ 3, 22].
A manual curation of these discordant cases suggested that these could be due to paralogous genes being sequenced, although they could also be caused by sequencing errors.
More suggestions(17)
be affected by sequence
be caused by nucleotide
be caused by chemical
be misled by sequence
be classified by sequence
be caused by blood
be caused by kidney
be queried by sequence
be searched by sequence
be marked by sequence
be caused by volume
be caused by pollen
be addressed by sequence
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be confounded by sequence
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be done by sequence
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