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These differences may be caused by sample size or study design.
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One possible explanation may be caused by sampling bias.
A portion of the strand bias can also be caused by sampling variation during the sequencing.
In addition, the AUC for CEA in our study is higher than those in the literature where they range from 0.69 to 0.79 [ 28– 31], which might be caused by samples in advanced stages or those including distant metastases.
A major weakness of these too promising results is that this report lacks a real true-negative group of patients because negative TRUS-guided biopsy results do not imply that no locally recurrent PCa is present as a false-negative result may be caused by sampling error.
At shorter acquisition times, the image resolution was similar or reduced, which rules out that the resolution reduction at longer acquisition times was caused by sample or defocus drifts.
40 events with irregular mtDNA patterns were detected: eight instances (from seven different haplotypes) could be traced to laboratory personnel; six cases were caused by sample cross-contamination.
Race-related differences were caused by samples from younger African Americans, while results obtained with samples of aged African Americans were similar to those of aged Caucasians.
Inflated distances at a low sampling depth are caused by sampling variation especially for the rare lineages.
This last category was expected to contain pairs of monozygotic twins based on the SNP information, but may have been caused by samples being mixed up (e.g. allocating two samples of one animal to two different pedigree entries).
These inconsistent findings might be caused by different samples, small sample sizes and methodological differences.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com