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In addition to the illustrated nanobioconjugation strategies, we emphasize that all these features need to be carefully optimized when assessing the biological effects of synthetic nanoparticles designed for biomedical purposes.
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For AIA detection the aspirated volume of sample and reagents should be carefully optimized.
The tissue contrast is strongly affected by the lighting conditions and must be carefully optimized.
To get meaningful results in these studies, the AsFlFFF conditions had to be carefully optimized.
However, primary cells of different origins respond differently, and methods need to be carefully optimized for each cell type.
The balance between the imaging dynamic range and system sensitivity may be carefully optimized depending on the sample scattering properties.
Due to the unknown quantity(ies) of candidate ligand(s) in each mixture sample, PMFS composition ratios have to be carefully optimized.
However, to overcome matrix effects and interfering matrix peaks, eluents, the chromatographic gradient, and the dilution factor need to be carefully optimized [ 14, 16].
Experimental conditions were carefully optimized for this assay strategy.
UPLC-MS/MS operation parameters were carefully optimized for the determination of LND.
Other parameters that could affect the CL intensities of the PCs were carefully optimized.
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