For AIA detection the aspirated volume of sample and reagents should be carefully optimized.
However, for maintaining high degree of specificity and sensitivity, thickness and coverage of Au immobilization layer can be carefully optimized to minimize TCE-induced deflection.
Thus, the flow rates and the DHE surface area should be carefully optimized in order to extract maximum heat from the reservoir with the minimum pressure losses.
The tissue contrast is strongly affected by the lighting conditions and must be carefully optimized.
To get meaningful results in these studies, the AsFlFFF conditions had to be carefully optimized.
However, primary cells of different origins respond differently, and methods need to be carefully optimized for each cell type.
The balance between the imaging dynamic range and system sensitivity may be carefully optimized depending on the sample scattering properties.
Due to the unknown quantity(ies) of candidate ligand(s) in each mixture sample, PMFS composition ratios have to be carefully optimized.
However, to overcome matrix effects and interfering matrix peaks, eluents, the chromatographic gradient, and the dilution factor need to be carefully optimized [ 14, 16].
Experimental conditions were carefully optimized for this assay strategy.
