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The work shows how the data in this task may be captured using either action-centered knowledge alone or both action-centered and non-action-centered knowledge, though the combined approach produced a better fit.
This type of kinetic behavior cannot be captured using either stoichiometric models or a direct flux comparison between flux states.
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In this work, we designed and developed an efficient, fully automated, direct matching-based FR approach, that is designed to operate efficiently when face data is captured using either visible or passive infrared (IR) sensors.
Images were captured using either Leica LAS AF (Leica Microsystems, Milton Keynes, UK) with a Leica DFC420C camera (Leica Microsystems, Milton Keynes, UK) or Zeiss LSM Image Browser (Carl Zeiss, Welwyn Garden City, UK).
Mounting was performed using Vectashield mounting medium (Vector Laboratories) and images were captured using either a Zeiss LSM510 confocal microscope.
Digital images of stained embryos were captured using either the Advanced Color SPOT system or the Retiga EXi Fast 1394 mounted onto a Nikon E600 compound microscope.
Fluorescent images were captured using either a Zeiss Axioskop 20 fluorescence microscope (Zeiss, Luton, UK) using Smartcapture software (Digital Scientific, Cambridge, UK) or a Zeiss Axioplan II fluorescence microscope (Zeiss, Luton, UK) using IPLab Spectrum software (Scanalytics Corp ,Fairfax, VA).
Images were captured using either an Axiocam HRc camera (Carl Zeiss, Jena, Germany) or a KY-F75 camera (JVC, Japan).
Images were captured using either an inverted fluorescence (Olympus IX71) or confocal (Zeiss LSM510) microscope and processed using Volocity and MetaFluor software.
Images were captured using either a Leica DMI6000-AF6000 fluorescence microscope or a Leica SP5 confocal laser-scanning microscope (both from Leica Microsystems).
Images were captured using either a Nikon CoolPix 995 Camera on a Leica M400 Photomicroskop or a Nikon D5000 camera on a Wild stereomicroscope.
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CEO of Professional Science Editing for Scientists @ prosciediting.com