Recent studies report that clathrin- or caveolin-mediated endocytosis is capable of internalizing particles with diameters upward to 500 nm, yet internalization efficiency decreases with increased size.
We showed that CD34 was found in ~50% of DNA-internalizing cells, and that 40 90% of CD34+ cells were capable of internalizing TAMRA-DNA.
Together these results indicate that a variety of APC populations in the PP and spleen are capable of internalizing CPMV, and the interaction with CPMV is consistent with display of the CPMV-binding protein, surface vimentin.
Despite the presence of several receptors that are capable of internalizing apoE such as low-density lipoprotein receptor (LDLR), LDLR-related protein (LRP), apoER2, and VLDLR, in neurons apoE is mostly imported via the LDLR [12] [15].
The conjugate was capable of internalizing into HUVEC and Saos-2 cells as demonstrated by colocalization of 82% of the conjugate with transferrin in HUVEC cells (Figure 3L O) and of 71% in Saos-2 cells (Figure 3P S).
Our experiments show that 1.5 7% of ascites cells (depending on the experiment and individual animal variation) are capable of internalizing the fragments of extracellular dsDNA.
It has been established previously that up to 40% of mouse CD34+ hematopoietic stem cells are capable of internalizing exogenous dsDNA fragments both in vivo and ex vivo.
