However, water-mediated hydrogen bonds and a unique cation-π sandwich stacking allow 447-52D to be broadly reactive with V3 containing both the GPGR and GPGQ crown motifs, while the deeper binding pocket and a buried Glu in the binding site of 537-10D litst its reactivity to only V3 containing the GPGR motif.
The V1V2 vaccine-induced Abs were shown to be broadly reactive with the V2 region of multiple subtypes despite immunization with immunogens from only subtypes B and CRF01_AE (Zolla-Pazner et al., 2013, Zolla-Pazner et al., 2014).
We demonstrate that our pan-papillomavirus CODEHOP assay is broadly reactive with all major branches of the virus family and show its utility in identifying a novel non-human primate papillomavirus in cynomolgus macaques.
Polyclonal antibody (Meridian Diagnostics, Cincinnati, OH, USA) used in this assay is broadly reactive with multiple fungal species, including B. dermatitidis, making it useful for detection, but not speciation, of yeasts in tissues.
IHC assays that used a polyclonal anti– N. meningitidis group Y antiserum that is broadly reactive with serogroups A, B, C, W, and Y, and a specific monoclonal anti– N. meningitidis serogroup C antibody, revealed immunostaining in the leptomeninges, lung, and skin tissues (3, 4 ).
MAb 3D9 was subsequently shown to be broadly cross-reactive with other flaviviruses, consistent with binding near the highly conserved fusion loop.
Early in the development of aCL immunoassays, it was observed that most APS-associated anticardiolipin antibodies appeared to be broadly cross-reactive with other anionic phospholipids, such as phosphatidylserine, phosphatidylinositol, phosphatidic acid, and phosphatidylglycerol.
The V2 mAbs target conserved epitopes, including the binding site for α4β7 integrin, and are broadly cross-reactive with various gp120 proteins.
However, because SNV is broadly cross-reactive with most New World hantavirus, the specific hantavirus cannot be identified.
Determining the incidence of dengue in travelers is hampered by the fact that dengue immunoglobulin (Ig) G antibodies are broadly cross-reactive with other flaviviruses and vaccines against them (e.g., yellow fever and Japanese encephalitis).
