By combining the methods of separation of variables and the Laplace transform, the exact solution of the DPL model of heat conduction in a three-dimensional bounded domain has been obtained and analyzed.
Each of the unbound and bound fractions was pooled and analyzed for electrophoresis.
After washes, bound proteins were eluted and analyzed by SDS-PAGE.
The bound proteins were eluted and analyzed by Western blot with polyclonal antibodies against TR α1 or DOR.
After washing the beads thrice by centrifugation, the bound proteins were eluted and analyzed by 10% SDS-PAGE followed by Western blotting probing for LASP-1 with mouse monoclonal anti-HA antibody (Sigma, St . Louis MO).
After incubation, bound proteins were eluted and analyzed by SDS-PAGE and silver staining.
The un-bound fraction was collected and analyzed for cellulase activity and protein content.
The co-immunoprecipitated BmAgo2-bound RNAs were extracted and analyzed by PAGE.
In the first set of simulations, repositioning time courses at a single fraction of bound ISWI were generated and analyzed.
In all cases, after extensive washing, bound proteins were eluted and analyzed by Western blotting with anti-p30 monoclonal antibody (diluted 1 500) or anti-p54 serum (diluted 1 60).
