Incubation of 35S-Met labeled WT Ey with immobilized glutathione S-transferase (GST) or GST tagged Ey deletion proteins EyN, PD and HD (Fig. 4A) revealed that WT Ey (WT*) was bound specifically to all three of the GST-Ey deletion proteins (Fig. 4B, WT*).
The same analysis method was used to analyze sequence reads from all samples, and thus the sites in which editing is responsive to gene knockdown, or that are bound specifically to ADAR proteins, cannot be artifacts of computational analyses.
Prior to adsorption of apododecin tE, a non-binding dodecin variant dA was added as a negative control to show that apododecin tE is bound specifically to the flavins.
Very limited numbers of infected cells were present outside the biotinylation areas, indicating that biotinylated Ad5.CMV-LacZ was bound specifically to biotin moieties on the well surface via Neutralite avidin.
The amount of I-labeled IGF-1 remaining in cartilage in the presence of 11.7 nM IGF-1 was 53% of the control value, demonstrating that only 47% of the radiolabeled peptide was bound specifically to proteins within cartilage (Fig. 7).
SDF1 or CXCL12 is a chemokine which binds specifically to the G-protein coupled receptor, CXCR4 [1].
The signal functions of MBD2 are to bind specifically to methylated gene promoters and recruit histone deacetylases and chromatin remodeling proteins.
The proposed mechanism of action of osmotin, a homologue of adiponectin, is to bind specifically to the receptor domain of Izh2p which causes a signal cascade that triggers PCD [ 17].
The reasoning behind this is that SeqA has been shown to bind specifically to hemimethylated DNA "trailing" the replication fork.
The TFIID subunit TAF3 has been shown to bind specifically to H3K4me3-modified nucleosomes that is also enhanced by coinciding H3K9/14ac [ 23].
