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These conjugates could be bound simultaneously by their relevant anti-steroid antibody and NeutrAvidin, and their linkers were easily cleaved by dithiothreitol (DTT) treatment.
Molecular modeling shows that the ETS⇔CRE motif can be bound simultaneously by both the ETS and the B-ZIP domains without protein-protein clashes.
More recently, the molecular basis of FAPP1-PH binding to ARF and PtdIns4 P has been elucidated in detail leading to the concept that both ligands can be bound simultaneously and independently to determine its multivalent anchoring to TGN membranes [14].
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One molecule of elaidoylamide is bound simultaneously to the substrate binding sites of two associated neurotoxic phospholipase A2 molecules.
These predictions show that there is virtually no Ste5 that are simultaneously bound to two kinases, such as Ste11 and Ste7 or Ste7 and Fus3 since on average there is usually less than one Ste5 molecule that is bound simultaneously to two of the kinases at a given time.
It is, therefore, feasible that MOP and NPN were bound simultaneously, and given the vicinity between the two ligands MOP could cause quenching of NPN fluorescence.
Occupancy levels for both GE and RifSV were 1, indicating that GE and RifSV were bound simultaneously to RNAP in the crystal.
The latter format has been examined by making a vaccine in which five different pneumococcal antigens were bound simultaneously to a single BLP carrier (van Roosmalen and Leenhouts, unpublished results).
The nearly complete loss of binding to R9G/H10Y and S104E indicates that both regions are bound simultaneously, consistent with the distances between the amino acids and the observed maximum dimensions of single epitopes of up to 21 × 28 Å.
Although we cannot rule out the possibility that both factors are bound simultaneously to these promoters, analysis of Ndt80 and Sum1 protein levels suggests that at 9 hours mainly Ndt80 binds whereas at 15 hours mainly Sum1 is bound to the promoters.
Finally, our observation that a single nucleosome can accommodate up to two bound ISWI enzymes raises several questions regarding the nucleosome repositioning activity of such a complex: if two ISWI enzymes were bound simultaneously to the same nucleosome, would only one or both be active during repositioning?
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