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If all three replicates were not-detected, then the quantity of bacteria present was assumed to be below the limit of detection.
Use of FPs also has the advantage of increased sensitivity at low cell densities, where other reporters may be below the limit of detection.
For example, the sRNA transcript level could be below the limit of detection of our Northern protocol, or there could be an absence of inducing signal for sRNA transcription prior to RNA isolation.
For example, in a hypothetical population in which the prevalence of viral suppression increases from 60% to 80% over 5 years, median viral load would be below the limit of detection every year and thus unchanged.
Also, considering that even addition of a small number of CP-AMPARs is predicted to alter synaptic transmission [41], the changes may be below the limit of biochemical detection.
However, quantification of correct message formation using SyberGreen quantitative PCR showed expression of LEDGF/p75 to be below the limit of detection in the −/− cells, corresponding to a reduction of at least 32-fold compared to the wild type cells (unpublished data).
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The fluorescent signal is below the limit of detection in shr-2 plants.
The cladding peak temperature of the PRHRS reached 562 °C, which was below the limit (750 °C).
The radiography technologists' average exposure during these procedures was below the limit of detectablility.
The alliinase protein levels in #6 and #10 bulbs were below the limit of quantitation of the ELISA (Fig. 6A).
The Senate bill would allow people to certify, under penalty of perjury, that their assets were below the limit.
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