The Trump administration says the military construction projects that lose funding for the wall would be "backfilled" in the coming year's budget, as long as Congress passes the request in full and on time, and therefore none of the projects ultimately would be delayed or scrapped.
The street dealers they were arresting were being backfilled in less than 24 hours.
After Governor Bob Graham initiated the Save Our Everglades campaign in 1983, the first section of the canal was backfilled in 1986.
But, he added, "I was assured that the space in Lower Manhattan would be backfilled by high-paying jobs over the next several years".
A fire-polished, pulled glass pipette (0.5 mm ID, 1.0 mm OD, Sutter) was backfilled with a TexasRed dye (lysine-fixable 3000 MW, Invitrogen) dissolved in saline.
Micropipettes were backfilled with internal patch solution containing (in mM) K-gluconate 110, KCl 10, HEPES 10, Phosphocreatine 10, Mg-ATP 4, Na-GTP 0.3, Biocytin 0.1%, pH 7.3, with and osmolarity of 280 290 mOsm.
In (g ), insect neurons were backfilled with cobalt but not subsequently silver intensified.
The labeled neuronal preparations shown in Figures 1, 2 and 4 were obtained from grasshopper (Schistocerca americana) neurons located in abdominal ganglia and were backfilled with cobalt chloride and processed with the Timm's silver intensification procedure (see references below for protocols and recipes).
Each well was backfilled with the required volume up to 40 nl of 100% DMSO to ensure a final 1% DMSO concentration in the assay.
