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Radioactivity was automatically quantified by an electronic radiography system (Instantimager, CT).

The number of colonies was automatically quantified by computer based image software (Image J).

The nuclear localization of p65 was automatically quantified by using the Enhanced-Translocation module of MetaXpress (Molecular devices, Sunnyvale, USA).

Transferrin uptake was automatically quantified by using the Multi-wave Length Cell Scoring module of MetaXpress (Molecular devices, Sunnyvale, USA).

For each set of experiments, the intensity of each spot was automatically quantified by the software and the background was subtracted from the signal for each spot.

The infarcted myocardium was automatically quantified from the short-axis LGE images according to a previously described method.

Fluorescence intensity data from each array were automatically quantified using BlueFuse for microarrays (version 3.3) software (Bluegnome).

Stain intensity was automatically quantified.

Finally, the area ratios of collagen and elastic fibers were automatically quantified.

During transient hypoxia-ischaemia, cerebral energetic changes were monitored every 2 min by phosphorus-31 (P) MRS and the β-nucleotide triphosphate (NTP; mainly ATP) peak height was automatically quantified.

Figure 2D shows the evolution over time of several root traits that can be automatically captured by GLO-RIA (depth, width, area) and others that were manually quantified (primary root growth rate or number of lateral roots per primary root).