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However, there still remain one half of those differences that can be attributed to sequencing errors.
Therefore, the observed sequence variations detected within mature miRNAs can be attributed to sequencing errors and/or to sequence variations between the query and the reference genome.
This 1.25-fold variation among environments can be considered a threshold for detecting stoichiometric differences that cannot be attributed to sequencing bias.
Hence, the bulk of the uninformative marker loci in our data clearly cannot be attributed to sequencing error, because the probability of multiple identical errors at a specific nucleotide position at a given RAD site is practically zero.
This initial set of 92,814 SNPs and 15,060 InDels was then subjected to filters to eliminate the number of differences that might be attributed to sequencing errors.
The absence of these genes in the genome of S. mansoni, S. haematobium and other published genomes cannot possibly be attributed to sequencing gaps or annotation errors since the WGS sequencing approach is considerably reliable [ 49], and the fact that we adopted a multiple species approach covering the entire available sequenced genomes of all species makes this even more improbable [ 37, 40].
Similar(51)
Previously, low levels of intraisolate mtDNA variation (i.e. polymorphism) (Lee and Young 2009) and/or high rates of short indels in reads from 454-sequencing data (Formey et al. 2012) were attributed to sequencing errors.
In particular, our findings may explain why genome sequencing efforts have failed to register these sequence deviations or, if detected, why they may have been attributed to sequencing error and eliminated during curation.
The differences can be attributed to sequence effects rather than structural effects.
According to Goicoechea (2009), this seemingly controversial placement of Oryza brachyantha could be attributed to sequence conservation between AA and FF genomes in some of the loci under investigation.
The large branch found for Myotragus in the phylogenetic tree (Figure 3) could be attributed to sequence changes due to DNA damage or to an accelerated evolution of the Myotragus genome.
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