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Non-genetic (environmental) factors are also thought to influence predisposition, with ethnic, geographic, lifestyle and socioeconomic factors shown to be associated with variable risk of migraine [7].
The −156G>C SNP (rs352046) was chosen as our candidate SNP because of our previous work demonstrating this SNP to be associated with variable ENA-78 protein concentration in vivo [13].
High GC3 was previously found to be associated with variable expression, higher frequency of upstream TATA boxes, and an increase of GC3 from 5′ to 3′.
For this reason, we developed two multiplex assays that allow the genotyping of SNPs at these two genomic regions which have been shown to be associated with variable HbF levels in different populations.
The primary goal of this study was to develop a simple and rapid method to genotype SNPs in the BCL11A gene and in the HBS1L-MYB intergenic region that were shown in various studies to be associated with variable HbF levels in several populations.
TS 3′-UTR genotypes (6+/ 6+/, 6+/ 6− and 6−/ 6−) seem to be associated with variable TS mRNA levels (Mandola et al, 2004); however, the functional effect of the 3′-UTR polymorphism is not well defined yet (Calascibetta et al, 2004).
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But, syphilis was not found to be associated with variables such as level of CD4+ T-cell count, having multiple sexual partners, and current condom use practice.
Obsessive-compulsive disorder (OCD) is associated with variable risk of suicide and prevalence of suicide attempt (SA).
Differences in the length of the menstrual cycle (MCL) have been associated with variable female fecundity.
Treatment of hydrocephalus by shunting procedure is associated with variable outcomes, depending on the setting.
Recent studies have shown that these methodologies are associated with variable probabilities of liquefaction depending on the soil parameters.
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