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This is because short reads of repetitive elements align ambiguously and cannot be assigned to unique locations in the genome.
To enable multiplex sequencing of the libraries, we used a set of molecular identifying sequences (MIDs) ranging in length from 5 bp to 8 bp that allowed reads to be assigned to unique individuals.
Whilst we acknowledge that cell lines may be divergent from their ancestral tumour and not wholly representative of the full diversity of ovarian cancer, we believe our classification represents a foundation for further development, particularly since ovarian cell lines can be assigned to unique ovarian tumour subtypes and are not derived from any random scheme.
Similar(57)
Reads mapping to overlapping regions between multiple transcripts were assigned to unique transcripts based on information from the uniquely mapping portion of each transcript and by assuming an additive effect of the read depth in the overlapping regions.
Individual copies were assigned to unique clones through PCR analyses using locus specific primers (Figure 2).
In this method probes are assigned to unique gene identifiers, in this case Entrez IDs.
Using fluorescence in situ hybridization (FISH), 510 BAC clones were assigned to unique LG chromosome locations in this study.
EC numbers [ 28] were assigned to unique sequences that had BLASTX scores with a cut off value of E = 10-5 or less upon searching SWIR protein databases.
Enzyme commission (EC) numbers were assigned to unique sequences, based on the BLASTx search of protein databases, using a cut off E-value 10−5.
Enzyme commission (EC) numbers were assigned to unique sequences that had BLASTX scores with a cut-off value of E = 10-5 or less upon searching protein databases.
EC numbers were assigned to unique sequences that had BLASTX scores with an E value cut-off of 10-5 after searching the KEGG protein databases.
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