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After preprocessing and clustering of the 16S rRNA sequencing reads the resulting OTUs have to be assigned to reference sequences in the SILVA database.
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The query sequences were assigned to reference sequences according to the top BLAST hits (Table S 3).
As described earlier, reads were assigned to reference genomes based on a strict Blast identity cut-off (95%) identity.
When reads could be mapped to multiple reference locations, they were assigned to reference transcripts proportionally based on the relative number of unique reads already mapped to each of the reference sequences.
When reads could be mapped to multiple reference locations, they were assigned to reference transcripts proportionally based on the relative number of unique reads previously mapped to each of the reference sequences.
Therefore, the higher incidence of HZ that was observed in the rural areas could be partially attributable in part to a higher consultation rate among the rural population due to the lower number of patients that are assigned to reference GPs in rural practices.
Each project may be assigned to a reference, a term we use for the large-scale sequencing centers (e.g. the DOE Joint Genome Institute) or community species homepages (e.g. FlyBase).
A relatively large fraction of reads can be assigned to the reference genes of the gene catalogue created for both species, (on average 66.73% of the human samples to the human reference database, and 60.26% of the guinea pig samples to the guinea pig reference database).
Parthood relationships may be missing from such knowledge-bases altogether, and chemical descriptors may be assigned to entities without reference to generating software, experimental conditions, parameters, or data sources.
The Bayesian assignment test correctly assigned 20.1% of the individuals to their own source location (22.4 % being the proportion of individuals that could not be assigned to any of the reference populations).
Recently, Elling et al. [ 16] observed high diversity of multilocus genotypes (using a subset of the SSR loci scored in our study) in feral B. napus populations in northwest Germany; 58%percentt of the feral plants could be assigned to one of seven reference varieties.
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