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OGs can be assigned to functional classes by projecting the biological function of its individual proteins to the entire OG.
All absorption bands of the C-1s NEXAFS spectrum of fraction 3 can be assigned to functional groups of methanobactin.
Fourteen central subunits represent the minimal form of complex I and can be assigned to functional modules for NADH oxidation, ubiquinone reduction, and proton pumping.
Several proteins of the yeast LP could be assigned to functional groups such as phosphatidic acid biosynthesis, fatty acid activation, TG and SE metabolism and sterol synthesis.
Because overall changes in the expression of functionally related genes are more informative than the expression pattern of single genes, genes in microarray studies can be assigned to functional groups [ 16].
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Genes were assigned to functional categories (molecular function, biochemical process, cellular localisation, chromosome) using Gene Ontology (http://vortex.cs.wayne.edu/projects.htm; Draghici et al, 2003) and GenMAPP (www.genmapp.org) analysis (Dahlquist et al, 2002).
The 26 genes encoding proteins with discernible functions were assigned to functional categories according to a classification scheme adapted from Riley [ 19].
To better understand biologic functions enriched in the SAE transcriptome, the gene lists were assigned to functional categories using Gene Ontology molecular functions and the expression levels for ubiquitous and SAE-enriched genes were compared.
The predicted target genes were assigned to functional groups based on molecular function, biological processes and specific pathways.
Of these, 77,715 transcripts were assigned to functional categories of 'Biological Process' (48.5%), 27,187 to 'Molecular Function' (34.5%) and 55,346 to 'Cellular Component' (17%).
Proteins were assigned to functional categories (additional file 15) based on sequence homology or annotated function.
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