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Only four residues of that loop were assigned (G126, K128, L130, and D132) at pH 7.3, but only the first two could be assigned at pH 6.5.
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Protonation states were assigned at pH 7.5 using PROPKA (20).
It is interesting to note that three residues in the A B loop (L31, D35, and K38) show flexibility but unlikely correlated motion, but there is no evidence of any correlated motion in the G H loop, where only four residues were assigned at pH 7.3.
Previously, cases were assigned at random.
Seventy patients were assigned at random to an antibiotic protocol.
Statistical significance was assigned at P < 0.05.
Significance was assigned at p < 0.05.
Significance level was assigned at P<0.05.
Of the 138 residues that can yield HSQC signals, 120 resonances could be assigned in the spectrum at pH 3.5.
We eliminated residues whose N atoms were within 13 Å of iron (35 at pH 7.5 and 24 at pH 6.5) and those that could not be assigned or whose peak was overlapped with that of another residue, a combined total of 81 at pH 7.3 and 78 at pH 6.5.
Inflection at pH = ~8.5-9.2 can be assigned to pKa of deprotonation of RNA 3' hydroxyl - that is, of a general base.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com